Our antibody performance testing and antibody optimization services are especially intended for customers who need to get information of the antibody performance and sharp and high resolution images with report and protocols for marketing purpose. BioSiteHisto has a long experience with optimizations of antibodies with human and animal tissues. We have a selection of control tissues for optimization and can in addition optimize with customer delivered tissues.
Our antibody optimization service includes:
- Tissue sectioning
- Immunoperoxidase staining method
- BioSiteHisto multi-tissue sections or customer’s tissues for optimization
- Three different pretreatments (HIER pH9, HIER pH6 and no pretreatment) for paraffin sections
- Five different dilutions using different staining steps per antibody
- Possible IF performed according to the result of immunoperoxidase staining
- Imaging and reporting
Our antibody performance testing includes:
- Tissue sectioning
- Immunoperoxidase staining method
- BioSiteHisto multi-tissue sections or customer’s tissues for testing
- Pretreatment: HIER pH9 for paraffin sections
- Three different dilutions using different staining steps per antibody
- Possible IF performed according to the result of immunoperoxidase staining
- Imaging and reporting
![EPCAM [BS14] Optimization](https://biositehisto.com/wp-content/uploads/antibody-optimization-370x278.jpg){kind=link}